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New findings about trichodysplasia spinulosa–associated polyomavirus (TSPyV)—novel qPCR detects TSPyV-DNA in blood samples

Diagnostic Microbiology and Infectious Disease. 2016; 
Paulo R.UrbanoaLuiz H.S.NaliaCamila S.BicalhobLigia C.PierrottibEliasDavid-NetobCláudio S.PannutiaCamila M.Romanoa
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Gene Synthesis The optimal temperature for primers and probe annealing was 58 °C. Samples were tested in duplicates, and a plasmid containing only the region of interest was commercially acquired (GenScript CO) and used as positive control.  Get A Quote

摘要

A new real-time PCR assay for trichodysplasia spinulosa–associated polyomavirus(TSPyV) DNA detection was designed, and blood samples from kidney transplantrecipients and healthy individuals were screened. TSPyV-DNA was not detected in blood from healthy individuals, but 26.8% of kidney recipients presented TSPyV-DNA. This is the first report of TSPyV viremia.

关键词

BloodPolyomavirusReal-time PCRImmunocompromised patientTSPyV
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