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Anti-VHH抗体

驼科动物外周血中存在一种天然缺失轻链的重链抗体HCAbs（heavy-chain antibodies），其抗原结合位点仅由重链单链的可变区组成，克隆其可变区得到由一个重链可变区组成的单域抗体即为纳米抗体（sdAbs或VHHs）。VHHs的特殊结构克服了传统抗体开发周期长，稳定性较低，保存条件苛刻等缺点，逐渐成为生物医药与临床诊断试剂中的新兴力量。
GenScript基于专有杂交瘤细胞技术的MonoRab™技术平台，自主研发的抗纳米抗体系列产品，为研究人员鉴定目标特异性的纳米抗体提供了高性能的二抗工具。

: 高特异性
与小鼠，大鼠，山羊，兔，人等多物种免疫球蛋白无交叉反应

: 驼科 & 人源化VHH全能检测
可提供识别驼科，人源化VHH的抗体和识别范围更广的cocktail版本

: 稳定的单抗工艺
单抗工艺，批次间CV＜10%

应用场景

: VHH纳米抗体的筛选和效价测定

: 胞外域为VVH纳米抗体的CAR-T细胞的表达评估及分选

: VHH纳米抗体的纯化

: 丰富验证数据

快速下单

概览选型指南全部产品性能数据应用数据相关推荐资源下载联系我们

选型指南

Product Type	Species Specificity	Unconjugated	Conjugated
Product Type	Species Specificity	Unconjugated	HRP	Biotin	iFluor 488	iFluor 555	iFluor 647	PE	FITC
Anti-Camelid VHH, mAb	Llama, Camel, Alpaca	A01860	A01861	A01995	A01862	A01863	A01994	A02227	A02226
Anti-Humanized VHH, mAb	Humanized, Llama, Camel, Alpaca	A02165	A02167	A02166	A02168	A02169	A02170	A02171	A02172
Anti-Camelid VHH, mAb Cocktail	Llama, Camel, Alpaca	A02014	A02016	A02015	A02021	A02020	A02019	A02018	A02017

性能数据

MonoRab™ Rabbit Anti-Camelid VHH Antibody (Cat. No A01860)

Binding compatibility of A01860 with 20 different VHHs

Cross reactivity of A01860 with lgG of different species

ELISA binding of A01860 with different subclasses camelid lgG

ELISA of A01860 with denatured or native camelid IgG
MonoRab™ Rabbit Anti-Humanized VHH Antibody, mAb (Cat. No A02165 )

Binding compatibility comparison of MonoRab™ Rabbit Anti-Humanized VHH Antibody, mAb (Cat. No A02165 ), MonoRab™ Rabbit Anti-Camelid VHH Antibody, mAb (Cat. No A01860 ), and MonoRab™ Rabbit Anti-Camelid VHH Cocktail (Cat. No A02014 ) with humanized VHHs. The performance of A02165 is the best. It is ideal to be used for humanized VHH antibody detection.

MonoRab™ Rabbit Anti-Camelid VHH Cocktail Antibody (GenScript Cat.A02014)

Binding compatibility comparison of A01860 and A02014 with 17 different VHHs

The Affinity of A02014

Cross reactivity of A02014 with lgG of different species

ELISA binding of A02014 with different subclasses camelid lgG

Product Type	Species Specificity	Unconjugated	Conjugated
Product Type	Species Specificity	Unconjugated	HRP	Biotin	iFluor 488	iFluor 555	iFluor 647	PE	FITC
Anti-VHH, mAb	Llama, Camel	A01860	A01861	A01995	A01862	A01863	A01994	-	-
Anti-VHH, mAb Cocktail	Llama, Camel, Alpaca	A02014	A02016	A02015	A02021	A02020	A02019	A02018	A02017

应用数据

FACS

图2. 使用不同浓度的MonoRab™ Rabbit Anti-Camelid VHH Cocktail [PE]（GenScript，A02018）通过流式细胞术鉴定CAR-T细胞

随着CAR-T疗法的不断发展，VHH在构建CAR中的使用也越来越频繁。该抗体与基于VHH纳米抗体的Jurkat-CAR细胞表现出优异的结合活性，建议在CAR-T细胞验证和鉴定中使用该抗体
MACS

图3.MonoRab™ Rabbit Anti-Camelid VHH Cocktail [Biotin]（GenScript，A02015）在CART细胞磁分离上的应用

实验采用初始T细胞作为CAR阴性的细胞对照，以9.43% CAR阳性T细胞与初始T细胞混合作为阳性待分离样品，通过生物素标记的抗体MonoRab™ Rabbit Anti-Camelid VHH Cocktail [Biotin]（GenScript，A02015）和抗生物素的磁珠进行CART细胞的磁分离，将得到的细胞进行流式细胞分析，以验证磁分离的效果。如图所示，经过磁分离后，CAR阳性细胞的比例从预混的9.43%提升至分离后的88.9%，数据表明该抗体可以很好的应用于CART细胞的磁分离。
Sandwich ELISA Application

Standard curve of VHH Sandwich ELISA. The VHH Sandwich ELISA assay is developed by using MonoRab™ Rabbit Anti-Camelid VHH Antibody, mAb (GenScript, A01860) and MonoRab™ Rabbit Anti-Camelid VHH Cocktail [HRP] (GenScript, A02016) as the capture and detection antibodies, respectively.

In this ELISA assay, the detection antibody was labeled with horseradish peroxidase (HRP), if you needs an unconjugated antibody you can choose MonoRab™ Rabbit Anti-Camelid VHH Cocktail (GenScript, A02014). GenScript can provide customized conjugation services for this product per the customer's request.

The sensitivity of detecting VHH is up to 39 pg/ml.