| Products/Services Used | Details | Operation |
|---|---|---|
| Gene Synthesis | The Xcc flagellin conserved domain (Xflg22: QRLSSGLRINSAKDDAAGLAIS), based on GenBank accession number 21242719, was synthesized by GenScript USA Inc., Piscataway, NJ, USA. The Xflg22 solution was prepared by dissolving the lyophilized peptide in sterile distilled water to a final concentration of either 10 or 100 μm (Zipfel et al., 2004). Young, fully expanded leaves were used for the experiments. The Xflg22 solution was infiltrated into the abaxial surface of leaves using a 1‐cm3 insulin syringe with a needle until half of the leaf was saturated. Infiltration with distilled water was used as a control. Leaf tissue was collected at 0 (before infiltration), 6, 24, 72 and 120 h after infiltration, and RNA was subsequently extracted from infiltrated areas. Three different plants of each genotype were used as biological replicates. The experiments were repeated twice with similar results. | Get A Quote |
The bacterial agent of citrus canker disease (Xanthomonas citri ssp. citri, Xcc) has caused tremendous economic losses to the citrus industry around the world. Pathogen‐associated molecular pattern (PAMP)‐triggered immunity (PTI) is important to plant immunity. In this study, we compared the defence responses of citrus canker‐resistant and citrus canker‐susceptible genotypes to the Xcc‐derived PAMP flg22 (Xflg22) by analysing the expression of 20 citrus defence‐associated genes. We showed that, in the most resistant genotype, ‘Nagami’ kumquat, there was significant induction of several defence genes (EDS1, NDR1, PBS1, RAR1, SGT1, PAL1, NPR2 and NPR3) as early as 6 h and up to 72 ... More
