| Products/Services Used | Details | Operation |
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| Gene Synthesis | The extracted RNA was used as template to synthesise cDNA by using oligo d(T)18 and reverse transcriptase M-MLV (RNase H−) (Takara, Dalian, China) following the manufacturer's instructions. Afterward, the first-strand cDNA was amplified by PCR according to the following cycle conditions: one cycle of 94 °C for 3 min; 35 cycles of 94 °C for 45 s, 55 °C for 1 min and 72 °C for 45 s; and a final extension step at 72 °C for 10 min The amplified PCR products (approximately 230 bp) were purified and cloned into the PMD18-T vector (Takara, Dalian, China) and sequenced by GenScript Co., Ltd. (Nanjing). Subsequently, the 3′-untranslated region (UTR) of the gene was obtained by 3′ rapid amplification of cDNA ends (3′ – RACE) using 3′ – RACE amplification kits (Takara, Dalian, China) and sequenced as described above. For 3′ – RACE, specific primers were designed based on the nucleotide sequences of the cDNA already cloned (outer, 5′-CGGAAGAAGTTCAAGGAG ACGA-3′, and inner, 5′-AGCCGAACAAGAAGACAAGAAT-3′). | Get A Quote |
Kiwifruit is cold-sensitive and very susceptible to chilling injury (CI) during low temperature storage. In this study, kiwifruit (Actinidia chinensis cv. Hongyang) were pre-treated by water dip for 10 min at 20 (control) or 35, 45, or 55 °C (heat pretreatments) and then stored at 0 °C for 90 days to investigate the effect of hot water treatments (HWT) on chilling injury tolerance. Results showed that 35 °C and 45 °C HWT alleviated but did not completely prevent chilling injury development. By contrast, 55 °C HWT increased symptoms of chilling injury. The 45 °C HWT was the most effective at reducing chilling injury index and incidence. Compared with the other HWT, fruit treated at 45 °C exhibite... More
