| Products/Services Used | Details | Operation |
|---|---|---|
| Gene Synthesis | Differentially amplified fragments in T1 were removed directly from dried polyacrylamide gels, rehydrated with 50 μL of ddH2O, heated at 100 ºC for 5 min and slowly cooled down to room temperature. The tubes were centrifuged at 10000 r/min for 10 min. Aliquots of 5 μL supernatant were used as templates for reamplification. PCR reactions were performed with the same primer combinations and reaction conditions that were used for selective amplification. After verification using a 1.2% agarose gel, the band was recovered with a gel extraction kit (CWBIO, China) according to the manufacturer’s instructions. Subsequently, the product was ligated into the vector pUC18 (CWBIO, China) and transformed into the E. coli strain TOP10. Sequence determinations were performed by the GenScript Corporation (Nanjing, China). The homology search and sequence analysis were performed using the public database NCBI (http://www.ncbi.nlm.nih.gov). | Get A Quote |
Pepper seedlings were hydroponically cultivated in 0–20 mg/L extraneous lanthanum chloride (La3+) for different numbers of days to investigate its regulating effects under salt stress. The results showed that, depending on the salt stress of 0.75 g/L, a lower concentration of La3+ (1.0 mg/L) might result in the lowest death rate, increase POD activity and change the band type of the stem and root on the 8th day. Furthermore, a methylation analysis by the methylation-sensitive amplified polymorphism (MSAP) technique indicated that a lower concentration of La3+ (1.0 mg/L) triggered the lowest methylation level of 26.1%, of which the hypermethylation events were the primary type of methylation. In addition, the ... More
