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Transgenic mice convert carbohydrates to essential fatty acids

PLoS ONE. 2014; 
Victor J. Pai, #  Bin Wang, #  Xiangyong Li, Lin Wu, and Jing X. Kang  , *
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Gene Synthesis After modifying the gene sequence to optimize the codon usage, the fat-2 gene was synthesized by GenScript (Piscataway, NJ). The gene was synthesized to be flanked with EcoR I digestion sites and delivered on pUC57 plasmid. After amplification, the fat-2 sequence was inserted into a pCAGGS expression plasmid containing the chicken beta-actin promoter and cytomegalovirus enhancer (kindly provided by Dr. J Miyazaki, Osaka University Medical School). After ligation, the orientation was confirmed and the plasmid was amplified. Finally, the fragment containing the promoter, the fat-2sequence, and the polyA sequence was excised with SspI and Sfi I for microinjection (Figure S2). Get A Quote

摘要

Transgenic mice (named “Omega mice”) were engineered to carry both optimized fat-1 and fat-2 genes from the roundworm Caenorhabditis elegans and are capable of producing essential omega-6 and omega-3 fatty acids from saturated fats or carbohydrates. When maintained on a high-saturated fat diet lacking essential fatty acids or a high-carbohydrate, no-fat diet, the Omega mice exhibit high tissue levels of both omega-6 and omega-3 fatty acids, with a ratio of ∼1∶1. This study thus presents an innovative technology for the production of both omega-6 and omega-3 essential fatty acids, as well as a new animal model for understanding the true impact of fat on human health.

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