| Products/Services Used | Details | Operation |
|---|---|---|
| Gene Synthesis | The PCR amplification and sequencing of ITS1- 5.8S-ITS2 rDNA were conducted as described in Wen et al. (2012), and the primers ITS4 (5’-TCCTCCGCTTATTGATATGC-3’) and ITS5 (5’-GGAAGTAAAAGTCGTAACAAGG-3’) (White et al., 1990) were used. The PCR amplification and sequencing of nrSSU were conducted as described in Sung et al. (2007b), and the primers NS1 (5’-GTAGTCATATGCTTGTCTC-3’) and NS4 (5’-CTTCCGTCAATTCCTTTAAG-3’) (White et al., 1990) were used. In the amplification and sequencing of RPB1, we followed Castlebury et al. (2004); the primers CRPB1A (5’-CAYCCWGGYTTYATCAAGAA-3’) and RPB1Cr (5’-CCNGCDATNTCRTTRTCCATRTA-3’) (Castlebury et al., 2004) were used. All PCR products were sequenced by GenScript Biotechnology Co., Nanjing, China. | Get A Quote |
Cordyceps species are entomophagous pathogens with medicinal properties, mostly linked to cordycepin and N6 - (2-hydroxyethyl)-adenosine (HEA). An isolate of Cordyceps pruinosa (GZUCC 8552) was obtained from a fruiting body formed on the cocoon a Limacodidae insect collected in Guizhou Province, China. Morphological and molecular analysis (combined 5.8S ITS, RPB1 and 18S RNA) confirmed the species to be Cordyceps pruinosa. Metabolites of the isolate grown in liquid static and solid-state media were established by HPLC-MS. Cordycepin (5.311 mg/g) and HEA (0.558 mg/g) were produced by this strain. This is the first record of cordycepin from an isolate of Cordyceps pruinosa. As Cordyceps pruinosa is a good source ... More
