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| Gene Synthesis | The amino acid sequence, protein molecular weight, isoelectric point, stability index, and hydrophobicity of the BocCYP79B2 gene were analyzed and predicted by ExPASy (http://web.expasy.org) and NCBI (https://www.ncbi.nlm.nih.gov/). The prediction of protein secondary structure was done using DNAStar software. Subcellular localization was predicted by WoLF PSORT (http://www.genscript.com/wolf-psort.html). The conserved domain were predicted by NCBI (https://www.ncbi.nlm.nih.gov/Structure/cdd/wrpsb.cgi). Phylogenetic tree analysis of the CYP79B2 proteins was executed in MEGA 6.0 using the neighbor-joining (NJ) method. | Get A Quote |
CYP79B2 is an important cytochrome P450 monooxygenases in glucosinolate biosynthesis. Here, the Brassica oleracea var. capitata CYP79B2 (BocCYP79B2) gene sequences were obtained from Brassica database (BRAD), and preformed for bioinformatics analysis. The BocCYP79B2.1, BocCYP79B2.2 and BocCYP79B2.3 genes mapped to chromosomes 1, 3 and 7, and contains an open reading frame of 1,623 bp, 1,557 bp and 1,626 bp that encodes a 540, 518, 541 amino acid protein, respectively. Subcellular localization predicted all BocCYP79B2 genes were in the chloroplast. The conserved domain of the BocCYP79B2 protein is PLN02971. Homology analysis indicates that the CYP79B2 protein is apparently conserved during plant evolution. The f... More
