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Gene Synthesis> | The following primers were used in PCR reactions: 5’-TCTTTCTCACAGGCCGCATT-3’ (forward) and 5’-TGATTTCTCCACGGTCAGGC-3’ (reverse) for LncRNA uc063iod.1; 5’-GCACCGTCAAGGCTGAGAAC-3’ (forward) and 5’-TGGTGAAGACGCCAGTGGA-3’ (reverse) for GAPDH. The primers used were all synthesized by Genscript USA Inc. | Get A Quote |
Objective: To investigate the expression of LncRNA uc063iod.1 in patients with different diseases and to explore the correlation between expression level of LncRNA uc063iod.1 and Coronary Artery Disease (CAD). Method: Plasma samples were prepared using the blood extracted from total 101 patients undergoing coronary artery angiogram (63 CAD patients and 38 non CAD patients). The expression level of LncRNA uc063iod.1 was detected using quantitative real time polymerase chain reaction (qRT-PCR) and the relationship between expression level of LncRNA uc063iod.1 and CAD was explored. The clinicopathological features of patients with CAD were also discussed in our study. Results: The expression of LncRNA uc063io... More