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Isolation and characterization of Bacillus altitudinis JSCX-1 as a new potential biocontrol agent against Phytophthora sojae in soybean [Glycine max (L) …

springerlink. 2017; 
Xiaoxue Lu, Dongmei Zhou, Xi Chen, Jinfeng Zhang, Huiwen Huang & Lihui Wei
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Gene Synthesis Physiological and morphological characteristics of the JSCX-1 strain were identified according to Bergey’s Manual of Determinative Bacteriology. The tests included spore formation, gram staining, oxidase activity, catalase activity, gelatin liquefaction, starch hydrolysis and the citrate test. The genomic DNA of the strain JSCX-1 was extracted with Bacteria Genomic DNA Kit (CW Biotech, China). The 16S rRNA and gyrB gene sequences were amplified from the integrated chromosomal DNA of the isolate JSCX-1 using the universal primers 27F/1492R and UP-1S/UP-2Sr (Yamamoto and Harayama 1995; Galkiewicz and Kellogg 2008) (Table 1). The amplified fragments were sequenced by GenScript Co., Ltd. (Nanjing, China). The 16S rRNA and gyrB gene sequences were analyzed using BLAST network services at NCBI, and the genes with high similarities were selected as the references. Phylogenetic trees were constructed using MEGA version 5.1 (Tamura et al. 2011). Get A Quote

摘要

Physiological and morphological characteristics of the JSCX-1 strain were identified according to Bergey’s Manual of Determinative Bacteriology. The tests included spore formation, gram staining, oxidase activity, catalase activity, gelatin liquefaction, starch hydrolysis and the citrate test. The genomic DNA of the strain JSCX-1 was extracted with Bacteria Genomic DNA Kit (CW Biotech, China). The 16S rRNA and gyrB gene sequences were amplified from the integrated chromosomal DNA of the isolate JSCX-1 using the universal primers 27F/1492R and UP-1S/UP-2Sr (Yamamoto and Harayama 1995; Galkiewicz and Kellogg 2008) (Table 1). The amplified fragments were sequenced by GenScript Co., Ltd. (Nanjing, China). The 16S... More

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