| Products/Services Used | Details | Operation |
|---|---|---|
| Gene Synthesis | The PCRs were performed in an automatic DNA thermocycler (Bio-Rad, Hercules, CA, USA) according to the previously described methods (Allosop et al. 1993; Yin 2000; Yin et al. 2008), and the PCR products were separated by 1.5% agarose gel electrophoresis to assess the presence of specific fragments indicative of the presence of Theileria. After that, the amplification products were sequenced by GenScript Corporation (Piscataway, NJ, USA), and representative sequences of the 18S rDNA genes of T. luwenshuni and T. uilenbergi were analyzed using Basic Local Alignment Search TooL (BLAST) from the National Center for Biotechnology Information (NCBI) (http://www.ncbi.nlm.nih.gov/Blast.cgi). The 18S rRNA gene sequences of Theileria spp. obtained from Chinese Kunming mice in this study were deposited in GenBank. | Get A Quote |
Theileria luwenshuni and Theileria uilenbergi are important tick-borne pathogens and cause substantial losses to the sheep industry in China. The improvement in detection techniques has allowed the identification of multi-homing parasitism in Theileria parasites. Herein we evaluated the experimental infectivity of T. luwenshuniand T. uilenbergi in Chinese Kunming mice by screening blood samples of experimentally inoculated mice by microscopic examination (ME) and PCR. T. luwenshuni infected Chinese Kunming mice and 20 mice inoculated with this parasite were positive by ME and PCR. In addition, T. uilenbergi infected mice and 20 mice inoculated with this species were positive by ME and PCR. However, ... More
