| Products/Services Used | Details | Operation |
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| Gene Synthesis | The nuclear ribosomal internal transcribed spacer (ITS) region, the universal fungal barcode marker, was amplified using the primers ITS1 and ITS4 [90]. Polymerase Chain Reaction (PCR) was performed with Taq 2X Master Mix (New England BioLabs, Maine, MA, USA). PCR cycling conditions for the ITS consisted of an initial denaturation step at 95 °C for 3 min, 35 cycles of 95 °C for 45 s, 52 °C for 45 s, 72 °C for 1 min and a final extension at 72 °C for 5 min. PCR products were purified with ExoSAP-IT (Affymetrix, Santa Clara, CA, USA) and sequenced with the PCR primers by Genscript, Piscataway, NJ, USA. The fungal stains were identified based on the search results of ITS sequences with BLASTn in GenBank as well as the morphological data. | Get A Quote |
The goal of this study is to explore a new self-healing concept in which fungi are used as a self-healing agent to promote calcium mineral precipitation to fill the cracks in concrete. An initial screening of different species of fungi has been conducted. Fungal growth medium was overlaid onto cured concrete plate. Mycelial discs were aseptically deposited at the plate center. The results showed that, due to the dissolving of Ca(OH)2 from concrete, the pH of the growth medium increased from its original value of 6.5 to 13.0. Despite the drastic pH increase, Trichoderma reesei (ATCC13631) spores germinated into hyphal mycelium and grew equally well with or without concrete. X-ray diffraction (XRD) and sc... More
