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Kiss1 mRNA and Its Protein Distribution in Preoptic and Arcuate Hypothalamic Nuclei in Pre-Pubertal Female Swamp Buffaloes

Pakistan Veterinary. 2018; 
Thuchadaporn Chaikhun-Marcou , Pongsiwa Sotthibandhu , Chanud Yanprapasiri , Suvarin Pavasutthipaisit and Siriwat Suadsong*
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Gene Synthesis Plasmid DNA, inserted with a section of ovine Kiss1 gene (GenBank accession no. DQ059506) at the length of 375 base pairs, was generated by GenScript, NJ, USA. Get A Quote

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The relationship between kisspeptin and GnRH releasing in many species has been studied including post-pubertal buffalo, but not in pre-pubertal buffalo. The aims of this study were to detect the localization of Kiss1mRNA and the distribution of kisspeptin protein in the preoptic (POA) and arcuate (ARC) hypothalamic nuclei of pre-pubertal swamp buffaloes. Brains were collected from ten pre-pubertal female buffaloes (

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