| Products/Services Used | Details | Operation |
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| Gene Synthesis | The endophytic bacterial DNA was extracted using FastDNATM SPIN KIT for soil (MP Biomedicals, Santa ANA, CA) using manufacturer protocol. The 16S rRNA gene was amplified using eubacterial universal primers targeting position 27F to 1491R and HotStarTaq® Mix (QIAGEN) under the following conditions initial activation step at 95°C for 15 minute, denaturation temperature of 95°C for 1 minute, annealing temperature of 56°C and extension at 72°C for 1 minute and final extension at 72°C for 10 minutes. The amplified 16SrRNA gene was purified using QUICK clean II Gel kit (Genscript USA inc.) and sequenced directly. The generated sequences obtained were aligned together with reference sequences generated through the blast program (http:ncbi.nim.nih.gov). | Get A Quote |
Introduction: Microorganisms are a preferred source of enzyme production due to their high production capability and low cost of production. Bacterial endophytes occupy unexplored sites hence they represent a new source of enzymes with diverse applications. Mangrove plants in Kenya have traditionally been used as medicinal plants due to their bioactive metabolites. However the enzymatic activity of mangrove plants associated endophytes has not been studied. Aims & Objectives: The study is aimed at bioprospecting for enzymes with potential biotechnological applications from mangrove ecosystems. Methods & Materials: Forty-two bacterial isolates were cultured and isolated from the leaves and roots of six mangrove ... More
