| Products/Services Used | Details | Operation |
|---|---|---|
| Gene Synthesis | The isolates were identified using PCR amplifying 16S rDNA. PCR products were sent to the Genscript Company and further confirmed by sequencing. The 16s rDNA sequences of these isolates were searched using the Nucleotide BLAST tool (BLASTn, http://www.ncbi.nlm. nih.gov/BLAST) and were submitted to GenBank. | Get A Quote |
The objective of present study was to examine the prevalence and antimicrobial resistance of Mannheimia haemolytica isolated from the dead goats and sheep with pneumonia in Jiangsu, China. These bacteria were isolated, identified, serotyped with multiplex PCR, characterized by MLST and evaluated for drug susceptibility. The overwhelming majority of isolates (19/21) from goats and 1 isolate from sheep were identified as serotype 2, while only 1 isolate (1/21) from goat characterised as serotype 1. MLST profiles of Mannheimia haemolytica isolates revealed that these were diversity and most of isolates belong to ST44 (7/21) and ST7 (6/21). There were high levels of resistance against streptomycin (48%) and mezloci... More
