| Products/Services Used | Details | Operation |
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| Gene Synthesis | Samples of algae were collected from local water sources near agricultural areas (Fig. 1) where blooms were observed. Colonies of algae were skimmed from the surface and stored in pond water. The colonies were first observed using bright field microscopy with a 40X dry objective and compared to standard images of ultracellullar structure in the Landcare Research online database [20]. Algae from that colony was then inoculated into Bristol Media (2.94 mM NaNO3, 0.17 mM CaCl2·2H2O, 0.3 mM MgSO4·7H2O, 0.43 mM K2HPO4, 1.29 mM KH2PO4, 0.43 mM NaCl). Algae were grown in a 12:12 h light dark cycle at 30 °C, 3% CO2, and light intensity of 50 μmol photons m−2 s−1 with daily agitation [21]. Isolated algae were genotyped by extraction of algicidal genomic DNA as described by Singh et al. and sequencing the 16S rRNA gene (GenScript) [22]. | Get A Quote |
A growing global population and industrialization have come at the cost of induced climate change and pollution of natural resources, resulting in formation of toxic algal blooms in fresh water sources. In the US alone, these blooms cost an estimated $1.5 billion dollars each year to remediate. Current methods to combat such blooms such as copper sulfate treatment are expensive, ineffective, and environmentally toxic, motivating development of biochemical algaecides as green alternatives. Our previous research led to identification of mutant polysaccharide lyase (PL) enzyme H208F derived from Stenotrophomonas maltophilia Smlt2602 (WT), which displays increased activity on the polysaccharide polyglucuronic aci... More
