| Products/Services Used | Details | Operation |
|---|---|---|
| Gene Synthesis | The primers for the selected genes were designed with GenScript software in https://www.genscript.com/ssl-bin/app/primer (Table 1). To evaluate the efficiency of the primers, standard curves encompassing from 8 - 10 to 102 copies were plotted using serial dilutions of each cDNA. The real-time PCR program was described as follows: 95°C for 2 minutes; 40 cycles of 95°C for 15 seconds, 60°C for 30 seconds, 72°C for 30 seconds; and then 72°C for 7 minutes. Melting curves were obtained at 80°C. Data acquisition was performed for every 0.2°C increase in temperature, with a 10 seconds step. Average CT was calculated by ABI StepOne devices and analyzed using relative expression software tool (REST) (version 2009) and SPSS software. | Get A Quote |
Background: Phenytoin is one of the most important chemical drugs used for the treatment of skin wound. Objectives: In the present study, the molecular effect of phenytoin on the expression of VEGF and TGF-β genes was investigated. Methods: This study was conducted on 30 male rats with approximately equal body mass. Skin wounds were generated with an area of 2 cm and a depth of 0.5 mm on the back of the necks. The rats were divided into two case and control groups (15 rats in each group). Topical phenytoin was administered to the two groups 2 times a day (1%) and Vaseline (as control group). Each of control and treated groups were divided into three subgroups and the rats were euthanized with chloroform on d... More
