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Acclimatization, Isolation and Identification of Two Tetrabromobisphenol A (TBBPA) Degradation Bacteria Isolated from an Anaerobic Sludge

Advanced Materials Research. 2014; 
Yubin Tang, Qunjie Xu and Yulin Min
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Gene Synthesis … PCR amplification conditions were as follows: initial denaturation at 94°C for 10 min, with 30 cycles of 94°C for 30 s, then 55°C for 30 s and 72°C for 1.5 min; and a final extension at 72°C for 10 min. Sequence determination was completed by GenScript Co. (Nanjing, China) … Get A Quote

摘要

Two different tetrabromobisphenol A (TBBPA) degradation bacteria were isolated from an anaerobic sludge after 100 days of acclimatization, named as NJUST20 and NJUST21. Both of them are able to degrade TBBPA at 30 °C and pH 7.0. The degradation rates of NJUST20 and NJUST21 are 62.85% and 42.28%, respectively, with the initial TBBPA concentration of 10.00 mg/L. According to analysis of the 16S rDNA sequences, NJUST20 should be classified as a species of genus Enterobacter and NJUST21 as a species of genus Serratia, for which the name Enterobacter sp. NJUST20 and Serratia sp.NJUST2 are proposed.

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