| Products/Services Used | Details | Operation |
|---|---|---|
| Gene Synthesis | … 115 of the templates20 All amplicons were cloned to pMD-19T (TaKaRa) and sequenced 116 by GenScript (Nanjing, China) For each gene, its three sequences from the homolog 117 fragment and the 5'- and 3'-RACE reactions were assembled To confirm that all these … | Get A Quote |
[3H]Muscimol binding was measured in the lumbar spinal cord of female rats by in vitro quantitative autoradiography. Ovariectomized rats were treated subcutaneously with either oil, estradiol benzoate (EB) or EB plus progesterone (P) in a regime known to reliably induce sexual receptivity. The level of [3H]muscimol binding was highest in laminae I-III and in the region around the central canal. Binding was lower in laminae IV-VI and was frequently undetectable in the ventral horn. There was a significant increase in the level of binding in laminae I-III after EB treatment. There was also a significant increase after treatment with EB+P in comparison to both the ovariectomized and EB-treated groups in this same ... More
