| Products/Services Used | Details | Operation |
|---|---|---|
| Gene Synthesis | … Table 1 Macromolecule-production information. Source organism, Homo sapiens. DNA source, Synthetic gene (GenScript). Cloning vector, pUC57. Expression vector, pET-28a (Merck Millipore). Expression host, E. coli BL21(DE3) … | Get A Quote |
Human fumarase (HsFH) is a well-known citric acid cycle enzyme and is therefore a key component in energy metabolism. Genetic studies on human patients have shown that polymorphisms in the fumarase gene are responsible for diseases such as hereditary leiomyomatosis and renal cell cancer. As a first step in unravelling the molecular basis of the mechanism of fumarase deficiency in genetic disorders, the HsFH gene was cloned in pET-28a, heterologously expressed in Escherichia coli, purified by nickel-affinity chromatography and crystallized using the vapour-diffusion technique. X-ray diffraction experiments were performed at a synchrotron source and the structure was solved at 2.1 Å resolution by molecular rep... More
