| Products/Services Used | Details | Operation |
|---|---|---|
| Gene Synthesis | ), and scrambled P39, with the same amino acid composition as P39 but with a different amino acid sequence, biotin-YCIDAPVDLYCA (GenScript), were synthesized and further processed for circularization by formation of a disulfide bond between two cysteines in positions 2 and 11, resulting in the formation of an 8–amino acid loop (Fig. | Get A Quote |
After being delivered by the bite from an infected mosquito, Plasmodium sporozoites enter the blood circulation and infect the liver. Previous evidence suggests that Kupffer cells, a macrophage-like component of the liver blood vessel lining, are traversed by sporozoites to initiate liver invasion. However, the molecular determinants of sporozoite-Kupffer cell interactions are unknown. Understanding the molecular basis for this specific recognition may lead to novel therapeutic strategies to control malaria. Using a phage display library screen, we identified a peptide, P39, that strongly binds to the Kupffer cell surface and, importantly, inhibits sporozoite Kupffer cell entry. Furthermore, we determined that ... More
