| Products/Services Used | Details | Operation |
|---|---|---|
| Gene Synthesis | An Arabidopsis thalliana HISTONE2B cDNA fragment followed by the sequence encoding for a poly-alanine linker and the Venus coding sequence was synthesized with XbaI and EcoRI sites (Genscript, Piscataway, NJ, USA) and cloned into pGreenII0179_35S-CaMV via XbaI and EcoRI giving rise to pGreenII0179_35S::H2B-Venus.... An mCherry fragment with poly-alanine linker was amplified by PCR from synthesized mCherry-LTI6a cloned into pUC57 (Genscript, Piscataway, NJ, USA), employing forward primer H2B_Cherry_F, 5’- GCGGCCGCTGCCGCTGCGGCAGCGGCCatggtgagcaagggcgagg-3’ and reverse primer H2B_Cherry_R, 5’-TTACTTGTACAGCTCGTCCATGC-3’, and subcloned into pBluescriptII SK(-) (Stratagene, La Jolla, CA, USA). | Get A Quote |
The outermost cell layer of plants, the epidermis, and its outer (lateral) membrane domain facing the environment are continuously challenged by biotic and abiotic stresses. Therefore, the epidermis and the outer membrane domain provide important selective and protective barriers. However, only a small number of specifically outer membrane-localized proteins are known. Similarly, molecular mechanisms underlying the trafficking and the polar placement of outer membrane domain proteins require further exploration. Here, we demonstrate that ACTIN7 (ACT7) mediates trafficking of the PENETRATION3 (PEN3) outer membrane protein from the trans-Golgi network (TGN) to the plasma membrane in the root epidermis of Arabidop... More
