| Products/Services Used | Details | Operation |
|---|---|---|
| Gene Synthesis | The 18C05-yakubaSNP-pBPSUw constructs were cloned by Gibson Assembly as described above, except for 18C05yakT1008C and 18C05yakT1482C sequences, which were synthesized and cloned by GenScript (https://doi.... The 18C05-ancestral sequences were synthesized and cloned by GenScript into pBPSUw AatII and FseI sites, except for the 18C05_AncG869A, 18C05_AncT670G and 18C05_Anc-7SNP sequences, which were cloned by us by Gibson Assembly into pBPSUw AatII and FseI sites using the 18C05_Ancestral_Gibson_forward and 18C05_Ancestral_Gibson_reverse primers (https:// doi. | Get A Quote |
Diverse traits often covary between species [1-3]. The possibility that a single mutation could contribute to the evolution of several characters between species [3] is rarely investigated as relatively few cases are dissected at the nucleotide level. Drosophila santomea has evolved additional sex comb sensory teeth on its legs and has lost two sensory bristles on its genitalia. We present evidence that a single nucleotide substitution in an enhancer of the scute gene contributes to both changes. The mutation alters a binding site for the Hox protein Abdominal-B in the developing genitalia, leading to bristle loss, and for another factor in the developing leg, leading to bristle gain. Our study suggests that mo... More
