| Products/Services Used | Details | Operation |
|---|---|---|
| Plasmid DNA Preparation | Materials and Methods Plasmid construction Previously published camel anti-CEA[11] and anti-CD16 [12] antibodies were synthesized (Genscript) and then linked to mutated human IgG1 Fc fragments, which the anti-CEA VHH was linked to the knob mutant (T366W), and the anti-CD16 VHH 4 was linked to the hole mutant (T366S, L368A and Y407V) [9],[10].... After blocking, primary antibody binding, and secondary antibody binding, chemi-luminescence was developed using TMB Reagent (GenScript Cat. | Get A Quote |
Bispecific antibodies have emerged as powerful therapeutic agents given their high specificity and ability to induce a potent immune response. Various bispecific antibody formats have been designed and studied regarding their applications in cancer therapy, though associated with issues of short half-life or manufacturing difficulties. Herein, a novel bispecific antibody, SS-Fc, was constructed by pairing 2 single-domain antibodies, anti-CD16 and anti-CEA, which were fused with CH3 "knobs into holes" mutations individually. SS-Fc was expressed and purified from E.coli. In vitro and in vivo experiments confirmed that SS-Fc can form a heterodimeric bispecific antibody when expressed and purified from E. coli. By ... More
