In the seed pod， pod shatter and seed dispersal properties are in part determined by the development of a longitudinally orientated dehiscence zone (DZ) that derives from cells of the gynoecial valve margin (VM). Transcriptional regulation of the MADS protein encoding transcription factors genes () and () are critical for proper VM identity specification and later on for DZ development. Current models of and regulation indicate that the transcription factors FRUITFULL (FUL) and REPLUMLESS (RPL) repress these genes in the developing valve and replum domains， respectively. Thus the expression of the genes is restricted to the VM. encodes a MADS-box containing transcription factor that is predicted to act through CArG-box containing -regulatory motifs. Here we delimit functional modules within the cis-regulatory region and examine the functional importance of CArG box motifs within these regulatory regions. We have characterized a 2.2kb region upstream of the translation start site that drives early and late medial domain expression in the gynoecium， as well as expression within the VM and DZ. We identified two separable， independent -regulatory modules， a 1kb promoter region and a 700bp enhancer region， that are capable of giving VM and DZ expression. Our results argue for multiple independent -regulatory modules that support expression during VM development and may contribute to the robustness of expression in this tissue. Additionally， three closely positioned CArG box motifs located in the upstream regulatory region were mutated in the context of the 2.2kb reporter construct. Mutating simultaneously all three CArG boxes caused a moderate de-repression of the reporter that was detected within the valve domain， suggesting that these CArG boxes are involved in repression in the valve.