LAGLIDADG homing endonucleases (LHEs) are a class of rare-cleaving nucleases that possess several unique attributes for genome engineering applications. An important approach for advancing LHE technology is the generation of a library of design ‘starting points’ through the discovery and characterization of natural LHEs with diverse specificities. However， while identification of natural LHE proteins by sequence homology from genomic and metagenomic sequence databases is straightforward， prediction of corresponding target sequences from genomic data remains challenging. Here， we describe a general approach that we developed to circumvent this issue that combines two technologies: yeast surface display (YSD) of LHEs and systematic evolution of ligands via exponential enrichment (SELEX). Using LHEs expressed on the surface of yeast， we show that SELEX can yield binding specificity motifs and identify cleavable LHE targets using a combination of bioinformatics and biochemical cleavage assays. This approach， which we term YSD-SELEX， represents a simple and rapid first principles approach to determining the binding and cleavage specificity of novel LHEs that should also be generally applicable to any type of yeast surface expressible DNA-binding protein. In this marriage， SELEX adds DNA specificity determination to the YSD platform， and YSD brings diagnostics and inexpensive， facile protein-matrix generation to SELEX.