Next-generation sequencing technologies enable the rapid identification of viral infection of diseased organisms. However， despite a consistent decrease in sequencing costs， it is difficult to justify their use in large-scale surveys without a virus sequence enrichment technique. As the majority of plant viruses have an RNA genome， a common approach is to extract the double-stranded RNA (dsRNA) replicative form， to enrich the replicating virus genetic material over the host background. The traditional dsRNA extraction is time-consuming and labour-intensive. We present an alternative method to enrich dsRNA from plant extracts using anti-dsRNA monoclonal antibodies in a pull-down assay. The extracted dsRNA can be amplified by reverse transcriptase-polymerase chain reaction and sequenced by next-generation sequencing. In our study， we have selected three distinct plant hosts: Māori potato (Solanum tuberosum)， rengarenga (Arthropodium cirratum) and broadleaved dock (Rumex obtusifolius) representing a cultivated crop， a New Zealand-native ornamental plant and a weed， respectively. Of the sequence data obtained， 31-74% of the reads were of viral origin， and we identified five viruses including Potato virus Y and Potato virus S in potato; Turnip mosaic virus in rengarenga (a new host record); and in the dock sample Cherry leaf roll virus and a novel virus belonging to the genus Macluravirus. We believe that this new assay represents a significant opportunity to upscale virus ecology studies from environmental， primary industry and/or medical samples.