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Enhanced transgene expression in rice following selection controlled by weak promoters.

BMC Biotechnol.. 2013; 
ZhouJie,YangYong,WangXuming,YuFeibo,YuChulang,ChenJuan,ChengYe,YanChenqi,ChenJian
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Gene Synthesis The -394 to +116 fragment of the original tCUP sequence [GenBank: AF133844] with the additional 3′ end Kozak consensus sequence (CCACCATGG; [32]) was synthesized commercially (Genscript, Nanjing, China). Get A Quote

摘要

Techniques that enable high levels of transgene expression in plants are attractive for the commercial production of plant-made recombinant pharmaceutical proteins or other gene transfer related strategies. The conventional way to increase the yield of desired transgenic products is to use strong promoters to control the expression of the transgene. Although many such promoters have been identified and characterized, the increase obtainable from a single promoter is ultimately limited to a certain extent.

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