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Single-cell analysis of long non-coding RNAs in the developing human neocortex.

Genome Biol.. 2016; 
LiuSiyuan John，NowakowskiTomasz J，PollenAlex A，LuiJan H，HorlbeckMax A，AttenelloFrank J，HeDaniel，WeissmanJonathan S，KriegsteinArnold R，DiazAaron A，LimDani
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Gene Synthesis Probes for in situ hybridization were synthesized (GenScript) or cloned from GW16 human fetal neocortex cDNA, which were reverse-transcribed using SuperscriptII (Invitrogen) with random hexamer primers. Get A Quote

摘要

Long non-coding RNAs (lncRNAs) comprise a diverse class of transcripts that can regulate molecular and cellular processes in brain development and disease. LncRNAs exhibit cell type- and tissue-specific expression， but little is known about the expression and function of lncRNAs in the developing human brain. Furthermore， it has been unclear whether lncRNAs are highly expressed in subsets of cells within tissues， despite appearing lowly expressed in bulk populations.

关键词

CRISPRi，Developing brain，Single-cell RNA-seq，ln
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