Understanding the metabolic mechanism of sterols to produce valuable steroid intermediates in mycobacterium by a noncoding small RNA (sRNA) view is still limited. In the work， RNA-seq was implemented to investigate the noncoding transcriptome of Mycobacterium neoaurum (Mn) in the transformation process of sterols to valuable steroid intermediates， including 9α-hydroxy-4-androstene-3，17-dione (9OHAD)， 1，4-androstadiene-3，17-dione (ADD)， and 22-hydroxy-23， 24-bisnorchola-1，4-dien-3-one (1，4-BNA).
Understanding the metabolic mechanism of sterols to produce valuable steroid intermediates in mycobacterium by a noncoding small RNA (sRNA) view is still limited. In the work， RNA-seq was implemented to investigate the noncoding transcriptome of Mycobacterium neoaurum (Mn) in the transformation process of sterols to valuable steroid intermediates， including 9α-hydroxy-4-androstene-3，17-dione (9OHAD)， 1，4-androstadiene-3，17-dione (ADD)， and 22-hydroxy-23， 24-bisnorchola-1，4-dien-3-one (1，4-BNA).
