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In vitro culture and morphological characterization of prepubertal buffalo (Bubalus bubalis) putative spermatogonial stem cell.

J. Assist. Reprod. Genet.. 2012; 
KalaS，KaushikR，SinghK P，KadamP H，SinghM K，ManikR S，SinglaS K，PaltaP，Chauha
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Gene Synthesis … Total RNA of cultured cells was extracted by the Trizol method (Invitrogen). First‐strand cDNA was synthesized from 10 μg total RNA by using the M‐MLV Reverse Transcriptase (GenScript Corporation, Piscataway, NJ, USA) … Get A Quote

摘要

Spermatogonial stem cells (SSCs) have the unique ability both to self-renew and to produce progeny that undergo differentiation to spermatozoa. The present study has been carried out to develop a method to purify and enrich the pure populations of spermatogonial stem cell like cells in buffalo.

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