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Gene Synthesis> | … with 60 pmols ITS4 and ITS5 primers[19], and 2.5 units Biotaq DNA polymerase. All the reaction reagents were provided by GenScript Corporation of China. The reaction was set up as follows: initial denaturation at 94°C for … | Get A Quote |
Traditional Chinese medicinal plants are sources of biologically active compounds, providing raw material for pharmaceutical, cosmetic and fragrance industries. The endophytes of medicinal plants participate in biochemical pathways and produce analogous or novel bioactive compounds. Panxi plateau in South-west Sichuan in China with its unique geographical and climatological characteristics is a habitat of a great variety of medicinal plants. In this study, 560 endophytic actinomycetes were isolated from 26 medicinal plant species in Panxi plateau. 60 isolates were selected for 16S rDNA-RFLP analysis and 14 representative strains were chosen for 16S rDNA sequencing. According to the phylogenetic analysis... More