| Products/Services Used | Details | Operation |
|---|---|---|
| Gene Synthesis | The luminescence was measured for 100 min after the proteins were added and either laminarin (Sigma Aldrich), chitin, flg22 (Genscript) or water were injected...Part of the FGB1 promoter was amplified using the primers 5′-AACGTGCGGCCGCTGAAT-3′ and 5′-CGATAGAGTTGGAGGCAAATC-3′ and fused to the remaining nucleotide sequence obtained from GenScript. | Get A Quote |
β-glucans are well-known modulators of the immune system in mammals but little is known about β-glucan triggered immunity in planta. Here we show by isothermal titration calorimetry， circular dichroism spectroscopy and nuclear magnetic resonance spectroscopy that the FGB1 gene from the root endophyte Piriformospora indica encodes for a secreted fungal-specific β-glucan-binding lectin with dual function. This lectin has the potential to both alter fungal cell wall composition and properties， and to efficiently suppress β-glucan-triggered immunity in different plant hosts， such as Arabidopsis， barley and Nicotiana benthamiana. Our results hint at the existence of fungal effectors that deregulate innat... More
