| Products/Services Used | Details | Operation |
|---|---|---|
| Custom Vector Construction | … The SA glucosyltransferase gene (ugt74f1) from A. thaliana (Gene ID:818988) was codon optimized and synthesized by GenScript (Piscataway, NJ, USA) and cloned into the pGEX-2TK vector with the BamHI and EcoRI restriction sites (Lee and Raskin, 1999; Lim et al., 2002 … | Get A Quote |
In this report， the heterologous production of salicylate (SA) is the basis for metabolic extension to salicylate 2-O-β-d-glucoside (SAG)， a natural product implicated in plant-based defense mechanisms. Production was optimized through a combination of metabolic engineering， gene expression variation， and co-culture design. When combined， SA and SAG production titers reached ~0.9g/L and ~2.5g/L， respectively. The SAG compound was then tested for anti-inflammatory properties relative to SA and acetylsalicylate (aspirin). Results indicate comparable activity between SAG and aspirin in reducing nitric oxide (NO) and reactive oxygen species (ROS) from macrophage cells while no discernable negative effe... More
