| Products/Services Used | Details | Operation |
|---|---|---|
| Gene Synthesis | … genes. The cDNAs encoding human CD55 (GenBank accession no. NM000574) and human CD59 (NM203330) were pur- chased from Origene, and the cDNA for human CD46 (NM002389) was synthesized by Genscript. Each … | Get A Quote |
Newcastle disease virus (NDV) is being developed as an oncolytic virus for virotherapy. In this study we analysed the regulation of complement-mediated inactivation of a recombinant NDV in different host cells. NDV grown in human cells was less sensitive to complement-mediated virus inactivation than NDV grown in embryonated chicken eggs. Additionally， NDV produced from HeLa-S3 cells is more resistant to complement than NDV from 293F cells， which correlated with higher expression and incorporation of complement regulatory proteins (CD46， CD55 and CD59) into virions from HeLa-S3 cells. Further analysis of the recombinant NDVs individually expressing the three CD molecules showed that CD55 is the most poten... More
