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Expression， purification and characterization of a thermostable leucine dehydrogenase from the halophilic thermophile Laceyella sacchari.

Biotechnol. Lett.. 2016-06; 
ZhuWenjun，LiYan，JiaHonghua，WeiPing，ZhouHua，Jian
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Plasmid DNA Preparation … The gene sequence of Ls-LeuDH was codon-optimized and synthesized by GenScript (Nanjing, China); it was generated by inserting the Ls-LeuDH gene into the XhoI–NdeI sites of pET-22b (+).The recombinant plasmid was transformed into BL21 (DE3) and the plasmids were … Get A Quote

摘要

A potential thermotolerant L-leucine dehydrogenase from Laceyella sacchari (Ls-LeuDH) was over-expressed in E. coli， purified and characterized.

关键词

Halophilic thermophile，Laceyella sacchari，Leucine dehydrogenase，Thermostable dehydroge
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