| Products/Services Used | Details | Operation |
|---|---|---|
| Gene Synthesis | … Applied Biosystems, Foster City, CA, USA) and 0.2 μM of reverse primer (IDT Inc., Coralville, IA, USA), 1× reaction buffer (20 mM Tris–HCl, pH 8.0, 50 mM KCl), 2.5 mM MgCl2, 0.2 mM of each of the dNTPs and 1 unit of Taq DNA polymerase (GenScript Corp., Piscataway, NJ … | Get A Quote |
Soybean cyst nematode (SCN， Ichinohe)， southern root-knot nematode [SRKN， (Kofoid and White) Chitwood] and reniform nematode (RN， Linford and Oliveira) are three important plant-parasitic pests in soybean. Previous study showed that plant introduction (PI) 567516C harbored novel quantitative trait loci (QTL) conferring SCN resistance to soybean. However， QTL underlying resistance to SRKN and RN in PI 567516C remain unknown. The objectives of this study were to identify QTL for resistance to SRKN and RN in PI 567516C. Two hundred and forty-seven F recombinant inbred lines， derived from a cross between cultivar Magellan and PI 567516C， were evaluated for resistance to SRKN and RN. Two hundred and... More
