| Products/Services Used | Details | Operation |
|---|---|---|
| Plasmid DNA Preparation | … ligase, according to the reference The gene phaPCJ (Accession: D888251) from A caviae (Fukui and Doi 1997) was synthesized by GenScript (Nanjing, China) and cloned into pUC57 To construct plasmid pBBJC, phaC from … | Get A Quote |
The copolymer poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) [P(HB-co-HHx)] has the potential to serve as a biodegradable tissue engineering material. However， the production of this kind of copolymer still suffers from high cost and uncertainty. We describe here the design of metabolic pathways to synthesize P(HB-co-HHx) directly from glucose using recombinant Escherichia coli. By combining the BktB-dependent condensation pathway with the inverted β-oxidation cycle pathway， we were able to synthesize a P(HB-co-HHx) copolymer with a 10 mol% HHx fraction in recombinant E. coli. After optimizing the host strain and employing thioesterase mutant strains， the engineered E. coli produced 12.9 wt% P(HB-co-HHx) ... More
