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Heterogeneous Nuclear Ribonucleoprotein A2/B1 as a Target Antigen in Han Chinese for BD Patients.

Protein Pept. Lett.. 2015; 
LiangJinghui，YangWeikang，MengXiangyu，ChenPeng，DuHo
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Gene Synthesis … The supernatant was carefully re- moved and stored after centrifugation in 12,000 rpm at 4 °C for 10 min Ni-chelating affinity chromatography purchased from GenScript (Qiagen, Hilden, Germany) was adapted to purify the product … Get A Quote

摘要

Behcet's disease (BD) is a recurrent pathema with a typical symptom of inflammation involved in many organs. Previous report indicated that the serum of Korean patients with BD stimulates membrane expression of hnRNP A2/B1 in endothelial cells. In this study， the target 35 kDa recombinant human hnRNP A2/B1 were over-expressed and purified， then sequenced with MALDI-TOF- TOF mass spectrometry. Western blotting and ELISA were applied to detect serum reactivity against hnRNP A2/B1 respectively. The results demonstrate that hnRNP A2/B1 is an autoantigen of BD in Han Chinese population.

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