Babesia spp. are tick-borne protozoan hemoparasites and the second most common blood-borne parasites of mammals， in particular domestic animals. We used the Clustal Multiple Alignment program and 18S rRNA gene sequences of 22 Babesia species from GenBank to develop a PCR that could detect a wide variety of Babesia spp. in a single reaction. The pan-Babesia FRET-qPCR we developed reliably detected B. gibsoni， B. canis， B. vogeli， B. microti， B. bovis， and B. divergens under controlled conditions but did not react with closely related species， mainly Hepatozoon americanum， Theileria equi， and Toxoplasma gondii.
Babesia spp. are tick-borne protozoan hemoparasites and the second most common blood-borne parasites of mammals， in particular domestic animals. We used the Clustal Multiple Alignment program and 18S rRNA gene sequences of 22 Babesia species from GenBank to develop a PCR that could detect a wide variety of Babesia spp. in a single reaction. The pan-Babesia FRET-qPCR we developed reliably detected B. gibsoni， B. canis， B. vogeli， B. microti， B. bovis， and B. divergens under controlled conditions but did not react with closely related species， mainly Hepatozoon americanum， Theileria equi， and Toxoplasma gondii.
