| Products/Services Used | Details | Operation |
|---|---|---|
| Gene Synthesis | … primers, made up to the final volume with ddH 2 O The thermal cycler conditions were 30 cycles of 95 °C for 30 s, 62 °C for 15 s, and 72 °C for 2 min 30 s All primers used in this study are shown in Table 2 and were synthesized by Genscript Biotechnology (Nanjing, China) … | Get A Quote |
Succinic acid synthesized from glucose shows potential as a bio-based platform chemical. However， the need for a high glucose concentration， and the accompanying low yields， limit its industrial applications. Despite efficient glucose uptake by the phosphotransferase system (PTS)， 1 mol of phosphoenolpyruvate is required for each mole of internalized glucose. Therefore， a PTS-defective Corynebacterium glutamicum mutant was constructed to increase phosphoenolpyruvate availability for succinic acid synthesis， resulting in a lower glucose utilization rate and slower growth. The transcriptional regulator iolR was also deleted to enable the PTS-defective mutant to utilize glucose via iolT-mediated glucos... More
