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Structural basis for the broad substrate specificity of the human tyrosylprotein sulfotransferase-1

Scientific REPORTS. 2017; 
Shinnosuke Tanaka, Toshiaki Nishiyori, Hidetaka Kojo, Reo Otsubo, Moe Tsuruta, Katsuhisa Kurogi, Ming-Cheh Liu, Masahito Suiko , Yoichi Sakakibara & Yoshimitsu Kakuta
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Gene Synthesis To circumvent potential complications associated with the crystallization of membrane proteins, we opted to overexpress the catalytic domain (Lys43–Glu370) of human TPST1 that lacks the N-terminal tail, the transmembrane domain and part of the stem region. cDNA encoding Lys43–Glu370 of human TPST1 was produced using total gene synthesis and cloned into pUC57 (GenScript, Piscataway, NJ). Get A Quote

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