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Transcriptomic analysis of Pak Choi under acute ozone exposure revealed regulatory mechanism against ozone stress.

BMC Plant Biol.. 2017; 
ZhangLu,XuBin,WuTao,WenMu-Xuan,FanLian-Xue,FengZhao-Zhong,PaolettiE
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Gene Synthesis The reverse transcription reaction system included 0.5 μL primer mix, 2μL RNA template, 0.5μL RT enzyme mix, 2 μL 5×RT buffer, 5μL ddH2O. In reference to the corresponding unigene sequence, gene-specific primers were designed using the online tool [GenScript Real-time PCR (TaqMan) Primer Design, https://www.genscript.com/ssl-bin/app/primer]. Get A Quote

摘要

Ground-level ozone (O) is one of the major air pollutants, which cause oxidative injury to plants. The physiological and biochemical mechanisms underlying the responses of plants to O stress have been well investigated. However, there are limited reports about the molecular basis of plant responses to O. In this study, a comparative transcriptomic analysis of Pak Choi (Brassica campestris ssp. chinensis) exposed to different O concentrations was conducted for the first time.

关键词

Glutathione metabolism,Ozone,Pak Choi,RNA-Seq,Transcrip