| Products/Services Used | Details | Operation |
|---|---|---|
| Gene Synthesis | ALKBH5 expression plasmid was generated by cloning the full-length ORF of human ALKBH5 gene (NM_017758.3) into pcDNA3.1-DYK vector (GenScript). ALKBH5 H204A was generated by GenScript. pcDNA3.1-DYK-HuR was generated by GenScript. ALKBH5 and HuR were cloned to pcDNA3.1 vector without tag. | Get A Quote |
The dynamic and reversible N-methyladenosine (mA) RNA modification installed and erased by N-methyltransferases and demethylases regulates gene expression and cell fate. We show that the mA demethylase ALKBH5 is highly expressed in glioblastoma stem-like cells (GSCs). Silencing ALKBH5 suppresses the proliferation of patient-derived GSCs. Integrated transcriptome and mA-seq analyses revealed altered expression of certain ALKBH5 target genes， including the transcription factor FOXM1. ALKBH5 demethylates FOXM1 nascent transcripts， leading to enhanced FOXM1 expression. Furthermore， a long non-coding RNA antisense to FOXM1 (FOXM1-AS) promotes the interaction of ALKBH5 with FOXM1 nascent transcripts. Depleting ... More
