| Products/Services Used | Details | Operation |
|---|---|---|
| Gene Synthesis | An E. coli codon optimized gene for PcyM_0533600 (Genscript, China) was synthesized and protein (Q30-K145) was expressed in BL21 cells. The protein was purified using a Ni-IMAC column followed by gel-filtration/buffer exchange and used to immunize rats (Eurogentec, Belgium). In addition, monoclonal antibodies were raised against selected proteins at Genscript, China. Primary antibodies were mouse mAb anti-H4K8ac (Active motif, Carlsbad, CA, USA, #61525, 1:500 dilution), polyclonal rat-anti-ETRAMP (PcyM_0533600, Eurogentec), mouse mAb 1G4E7 against GAP45 (PcyM_1442700, Genscript) and mouse mAb 5B10C7 against Ferredoxin (PcyM_1419800, Genscript). | Get A Quote |
liver hypnozoites， which cause disease relapse， are widely considered to be the last barrier towards malaria eradication. The biology of this quiescent form of the parasite is poorly understood which hinders drug discovery. We report a comparative transcriptomic dataset of replicating liver schizonts and dormant hypnozoites of the relapsing parasite . Hypnozoites express only 34% of physiological pathways， while 91% are expressed in replicating schizonts. Few known malaria drug targets are expressed in quiescent parasites， but pathways involved in microbial dormancy， maintenance of genome integrity and ATP homeostasis were robustly expressed. Several transcripts encoding heavy metal transporters were... More
