| Products/Services Used | Details | Operation |
|---|---|---|
| Gene Synthesis | Te amino acid sequence of CD1623 from C. difcile 630 was retrieved from NCBI and used to synthesize its encoding gene at GenScript Inc. USA, codon optimized for expression in E. coli; a truncated form containing only the two C-terminal domains (residues 401 to 843) was also synthesized and codon optimized for expression in E. coli. Both genes were cloned into a pET24a(+) vector at GenScript Inc. USA, utilizing the NdeI and HindIII restriction enzymes. | Get A Quote |
Flavodiiron proteins (FDPs) are enzymes with a minimal core of two domains: a metallo-β-lactamase-like， harbouring a diiron center， and a flavodoxin， FMN containing， domains. FDPs are O or NO reducing enzymes; for many pathogens， they help mitigate the NO produced by the immune system of the host， and aid survival during fluctuating concentrations concentrations of oxygen. FDPs have a mosaic structure， being predicted to contain multiple extra domains. Clostridium difficile， a threatening human pathogen， encodes two FDPs: one with the two canonical domains， and another with a larger polypeptide chain of 843 amino acids， CD1623， with two extra domains， predicted to be a short-rubredox... More
