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Streamlining recombination-mediated genetic engineering by validating three neutral integration sites in sp. PCC 7002.

J Biol Eng. 2017-01; 
VogelAnne Ilse Maria, LaleRahmi, Hohmann-MarriottMartin F
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Plasmid DNA Preparation … Plasmids were ordered from Genscript and named pUC57Simple_A2842, pUC57Simple_A0935 and pUC57Simple_A0159. A BioBrick compatible kanamycin resistance cassette was inserted as described in the BioBrick Standard Assembly protocols [28] … Get A Quote

摘要

sp. PCC 7002 (henceforth ) is developing into a powerful synthetic biology chassis. In order to streamline the integration of genes into the chromosome， validation of neutral integration sites with optimization of the DNA transformation protocol parameters is necessary. Availability of BioBrick-compatible integration modules is desirable to further simplifying chromosomal integrations.

关键词

BioBrick，Cyanobacteria，Genetic engineering，Neutral integration sites，Synechococcus sp. PCC7002，Synthetic biology，Transforma
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