| Products/Services Used | Details | Operation |
|---|---|---|
| Gene Synthesis | … A set of genus-specific and species-specific qPCR primers and TaqMan® hydrolyses probes (Table 1) were designed (Real-time PCR Primer Design, GenScript®, Piscataway, NJ, USA) with default param- eters using two sequences of S. parasitica (AB086899.1 and AB727993 … | Get A Quote |
Since 2010， the Loue River (Franche-Comté， East of France) has been suffering from massive fish kills infested by Saprolegnia parasitica. The river supplies inhabitants of the city of Besançon in drinking water， raising the question of a potential risk through both water consumption and use. We developed a real-time quantitative PCR (qPCR) to quantify S. parasitica in the Loue River as well as in the drinking water. A weak spatial trend is suggested with greater quantities of S. parasitica observed at the sampling station close to the main pumping station. No S. parasitica DNA was detected in the tap water connected to pumping stations. The use of qPCR， which combines specificity， practicality， sp... More
