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Transcriptome analysis of avian reovirus-mediated changes in gene expression of normal chicken fibroblast DF-1 cells

BMC Genomics. 2017-11; 
NiuXiaosai, WangYuyang, LiMin, ZhangXiaorong, WuYa
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Gene Synthesis … MIQE guidelines [38]. Briefly, 5 μg of total RNA (described above) was reverse transcribed using M-MLV reverse transcriptase (Transgen, Beijing, China) with a random hexamer primer (Genscript, Nanjing, China). The mixtures … Get A Quote

摘要

Avian reovirus (ARV) is an important poultry pathogen that can cause immunosuppression. In this study， RNA-Seq technology was applied to investigate the transcriptome-wide changes of DF-1 cells upon ARV infection at the middle stage.

关键词

Anti-viral responses，Avian reovirus，RNA-Seq，Transcrip
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