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Gene Synthesis> | … light. Sequencing and phylogenetic analyses. The secondary PCR products from the pigs were sequenced by Genscript Company (Nanjing, China). The accuracy of the sequences was confirmed by two-directional sequencing … | Get A Quote |
Cryptosporidiosis is caused by species of Cryptosporidium protozoa that can infect a wide range of host animals worldwide. However, data regarding Cryptosporidium infection in farmed pigs in subtropical areas in China is limited. Therefore, a total of 396 fecal samples were obtained from farmed pigs from Zhejiang (n = 124), Guangdong (n = 72), and Yunnan (n = 200) provinces, China, and were tested by PCR amplification of the small subunit (SSU) rRNA gene. The overall prevalence of Cryptosporidium in pigs was 17.68% (70/396), with 20.11% (36/179) in male pigs and 15.67% (34/217) in female pigs. Additionally, Cryptosporidium prevalence was 8.33% (6/72) in Guangdong province, 14.52% (... More